Agarose gel electrophoresis.

نویسندگان

  • Joseph Sambrook
  • David W Russell
چکیده

MATERIALS 6x Gel-loading buffer Agarose solutions (please see Step 3) DNA samples DNA size standards Samples of DNAs of known size are typically generated by restriction enzyme digestion of a plasmid or bacteriophage DNA of known sequence. Alternatively, they are produced by ligating a monomer DNA fragment of known size into a ladder of polymeric forms. DNA staining solution For a discussion of staining agarose gels, please see Detection of DNA in Agarose Gels. Electrophoresis buffer

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عنوان ژورنال:
  • CSH protocols

دوره 2006 1  شماره 

صفحات  -

تاریخ انتشار 2006